Laboratory khoom siv /kuaj cuab yeej yog tus khub zoo rau peb txoj haujlwm thiab yog qhov tseem ceeb hauv kev txiav txim siab qhov tseeb. Kev tu lawv tau dhau los ua ib txoj haujlwm nyuaj. Cov WUBOLAB pab neeg tau sau ntau txoj hauv kev hauv qab no.
Kev ntxuav cov iav tshiab
1. Txhuam nrog dej kais dej kom tshem tawm cov plua plav.
2. Kev ziab thiab soaking hauv hydrochloric acid: Qhuav hauv qhov cub, thiab tom qab ntawd rau hauv 5% dilute hydrochloric acid rau 12 teev kom tshem tawm cov av, hmoov lead, arsenic, thiab lwm yam.
3. Kev txhuam thiab ziab: yaug nrog dej kais dej tam sim ntawd tom qab 12 teev, tom qab ntawd txhuam nrog xab npum, yaug nrog dej kais dej thiab ziab hauv qhov cub.
4. Acid soaking thiab tu: soak hauv cov tshuaj ntxuav tu (120g poov tshuaj dichromate: 200ml concentrated sulfuric acid: 1000ml distilled dej) rau 12 teev, ces tshem tawm cov tais diav los ntawm lub acid tank thiab yaug nrog kais dej 15 zaug, thiab thaum kawg yaug nrog distilled. dej thiab ob chav dej distilled rau 3-5 zaug.
5. Kev ziab thiab ntim: qhuav tom qab ntxuav, thiab tom qab ntawd ntim nrog kraft ntawv (glossy paper).
6. Kev ua kom tsis muaj hluav taws xob siab: Muab cov khoom ntim ntim rau hauv lub tshuab ua kom siab, kaw lub hau, qhib qhov hloov thiab kev nyab xeeb valve, thaum lub zog nce hauv txoj kab ncaj nraim, kaw qhov kev nyab xeeb valve, thaum lub pointer taw tes rau 15 phaus, tswj rau 20-30 feeb.
7. Qhuav tom qab autoclaving.
Cov iav qub ntxuav
1. Kev txhuam thiab ziab: Cov iav siv tau tuaj yeem tsau ncaj qha rau hauv cov tshuaj Lysur los yog cov tshuaj ntxuav tes, thiab cov tais diav ntim hauv Sur tov (detergent) yuav tsum tau ntxuav nrog dej huv thiab ces qhuav.
2. Acid soaking thiab tu: Tom qab ziab, soak hauv cov tshuaj ntxuav (acid tov), tshem cov tais diav los ntawm lub tank acid tom qab 12 teev, thiab tam sim ntawd yaug nrog dej kais dej (kom tsis txhob muaj protein ntau adhering rau lub khob tom qab ziab thiab nyuaj. ntxuav), thiab tom qab ntawd siv dej distilled Yaug 3 zaug.
3. Kev ziab thiab ntim: Tom qab cov khoom siv ntxuav tau qhuav lawm, lawv tau muab tshem tawm thiab ntim nrog kraft daim ntawv (glossy paper) kom yooj yim kom tsis muaj menyuam thiab khaws cia thiab tiv thaiv cov plua plav thiab rov ua dua.
4. Kev kub siab ua kom tsis muaj menyuam: muab cov khoom ntim ntim rau hauv lub tshuab ua kom siab, kaw lub hau, qhib qhov hloov thiab kev nyab xeeb valve, qhov kev nyab xeeb valve yuav tawm cov pa thaum qhov kub thiab txias, thiab kaw qhov kev nyab xeeb valve thaum lub qhov cub tawm hauv txoj kab ncaj nraim. rau 3-5 feeb, Barometer Performance index nce raws li. Thaum tus taw tes taw qhia rau 15 phaus, kho qhov hloov hluav taws xob los tswj nws li 20-30 feeb. ( Maj mam npog lub hau yas ua ntej sterilizing iav kab lis kev cai khob).
5. Kev ziab kom qhuav: Vim tias cov tais diav yuav ntub los ntawm chav dej tom qab autoclaving, lawv yuav tsum tau qhuav rau hauv qhov cub kom sawv ntsug.
Cov khoom siv hlau tu
Cov khoom siv hlau yuav tsum tsis txhob ntub rau hauv cov kua qaub. Thaum ntxuav, koj tuaj yeem ntxuav lawv nrog xab npum ua ntej, tom qab ntawd yaug nrog dej kais dej, tom qab ntawd so nrog 75% cawv, tom qab ntawd yaug nrog dej, tom qab ntawd dej distilled, thiab tom qab ntawd qhuav lossis cua qhuav. Muab tso rau hauv ib lub thawv aluminium, ntim nws, thiab ua kom tsis muaj menyuam hauv lub qhov cub siab nrog 15 phaus siab siab (30 feeb), thiab tom qab ntawd qhuav kom siv tom qab.
Roj hmab thiab yas tu
Cov txheej txheem niaj hnub ua rau cov roj hmab thiab cov khoom lag luam yog: thawj zaug ntxuav nws nrog xab npum, tom qab ntawd yaug nws nrog dej kais dej thiab dej distilled, ces qhuav hauv qhov cub, thiab tom qab ntawd ua cov txheej txheem hauv qab no raws li qhov sib txawv:
1. Lub koob lim lub hau yuav tsum tsis txhob muab tso rau hauv cov kua qaub, soak nws nrog sodium hydroxide tov rau 6-12 teev, los yog boil rau 20 feeb. Ua ntej ntim, nruab ob lub lim membrane. Thaum txhim kho lub lim membrane, xyuam xim rau lub teeb sab (concave upward), ces loosen cov ntsia hlau me ntsis, muab tso rau hauv ib lub thawv aluminium, sterilize nws nyob rau hauv lub siab cooker rau 15 phaus rau 30 feeb, thiab qhuav rau tom qab siv. . Nco ntsoov tias cov ntsia hlau yuav tsum nruj tam sim ntawd thaum tshem tawm ntawm lub rooj zaum huv si rau kev siv. Rau
2. Tom qab cov roj hmab stopper qhuav lawm, boil nws nrog 2% sodium hydroxide tov rau 30 feeb (cov roj hmab stopper tsuas yog yuav tsum tau kho nrog boiling dej rau 30 feeb), yaug nrog kais dej thiab qhuav. Tom qab ntawd soak hauv cov tshuaj hydrochloric acid rau lwm 30 feeb, tom qab ntawd yaug nrog dej kais dej, dej distilled, thiab peb-distilled dej, thiab qhuav. Thaum kawg, muab tso rau hauv lub thawv aluminium rau autoclaving thiab ziab rau siv. Rau
3. Tom qab ziab, cov kaus mom yas thiab centrifuge raj lub kaus mom tsuas tuaj yeem tsau hauv 2% sodium hydroxide tov rau 6-12 teev (nco ntsoov tias lub sijhawm yuav tsum tsis txhob ntev dhau), ntxuav nrog dej kais thiab qhuav. Tom qab ntawd soak hauv cov tshuaj hydrochloric acid rau lwm 30 feeb, tom qab ntawd yaug nrog dej kais dej, dej distilled, thiab peb-distilled dej, thiab qhuav. Thaum kawg, muab tso rau hauv lub thawv aluminium rau autoclaving thiab ziab rau siv. Rau
4. Lub taub hau yas tuaj yeem soaked hauv 75% cawv rau 5 feeb, thiab tom qab siv UV irradiation. Rau
5. Lwm txoj kev tshuaj tua kab mob: qee yam khoom tsis tuaj yeem qhuav lossis ua kom tsis muaj menyuam, thiab tuaj yeem tua kab mob los ntawm soaking hauv 70% cawv. Qhib lub hau ntawm lub tais yas petri, muab tso rau ntawm lub rooj huv huv, thiab ncaj qha tso rau lub teeb ultraviolet kom tsis muaj menyuam. Ethylene oxide kuj tuaj yeem siv los tua cov khoom yas. Tom qab tshuaj tua kab mob, nws yuav siv sij hawm 2-3 lub lis piam los ntxuav cov ethylene oxide residual. Nws yog qhov zoo tshaj plaws los siv 20,000-100000rad r-ray kom tsis muaj menyuam cov khoom yas. Rau
Txhawm rau tiv thaiv kev tsis sib haum xeeb ntawm cov khoom siv tshuaj tua kab mob thiab tsis muaj tshuaj tua kab mob, tom qab cov ntawv ntim khoom, siv cov steganographic number case los ua cov cim. Tus txheej txheem yog siv tus cwj mem los yog txhuam los txhuam cov steganographic number case, thiab ua ib qho cim rau ntawm daim ntawv qhwv. Feem ntau, tus cwj mem no tsis muaj kab. Thaum qhov kub thiab txias, cov ntawv sau yuav tshwm sim, kom nws tuaj yeem txiav txim siab seb lawv puas muaj tshuaj tua kab mob. Kev npaj ntawm steganography number case: Chlorinated pob zeb diamond (CoC12•6H2O) 2g, 30% hydrochloric acid 10mL, distilled dej 88mL.
Ceev faj:
1. Ua raws li cov txheej txheem kev khiav hauj lwm ntawm lub siab cooker: Thaum autoclaving, thawj zaug xyuas seb puas muaj dej distilled hauv lub lauj kaub los tiv thaiv kom tsis txhob ziab hauv qhov kub siab. Tsis txhob muaj dej ntau dhau vim nws yuav thaiv cov huab cua ntws thiab txo cov nyhuv ntawm cov tshuaj tua kab mob siab. Xyuas seb qhov kev nyab xeeb valve puas tsis muaj kev cuam tshuam los tiv thaiv kev tawg ntawm kev kub siab.
2. Thaum txhim kho lub lim membrane, xyuam xim rau lub ntsej muag ci ntsa iab: nco ntsoov tias sab du ntawm lub lim membrane yog sab pem hauv ntej, thiab nws yuav tsum tig ntsej muag, txwv tsis pub nws yuav tsis tuaj yeem lim.
3. Ua tib zoo saib xyuas kev tiv thaiv ntawm tib neeg lub cev thiab kev ua tiav ntawm cov khoom siv: A. Hnav cov hnab looj tes acid-resistant thaum ntub cov kua qaub los tiv thaiv cov kua qaub los ntawm txaws thiab ua mob rau tib neeg lub cev. B. Thaum khaws cov tais diav los ntawm lub tank acid, tiv thaiv cov kua qaub los ntawm kev txaws rau hauv av, uas yuav corrode hauv av. C. Cov tais diav yuav tsum tau muab tso rau hauv cov kua qaub kom tiav, thiab tsis muaj cua npuas yuav tsum tau tso tseg kom tsis txhob ua kom tiav cov kua qaub.
