Nws yog ib qho kev ua lag luam zoo siab, muaj tswv yim thiab nkag siab yooj yim los pib lub chaw sim tshiab. Muaj 6 yam tseem ceeb uas yuav tsum tau xav txog thaum tsim lub chaw kuaj mob uas tuaj yeem pab nws ua tiav.
1. Lub Hom Phiaj ntawm Koj Lub Chaw Haujlwm Tshiab
Lub hom phiaj thiab kev ua haujlwm ntawm koj qhov chaw kuaj sim teeb tsa cov chav kawm rau cov dej num koom nrog hauv kev teeb tsa lub chaw kuaj mob tshiab.Cov khoom siv thiab cov txheej txheem yuav tsum sib txawv ntawm lub chaw kuaj mob uas siv los qhia lub hom phiaj thiab lub chaw tshawb fawb.
2. Lub koom haum
Thawj qhov uas koj yuav tsum tau ua yog xaiv qhov kev txiav txim siab meej uas haum rau koj qhov kev tshawb fawb xav tau los npaj nws cov haujlwm ua haujlwm, cov khoom muag, phau ntawv sau, thiab kev tshuaj xyuas cov txiaj ntsig rau ib chav kuaj tshiab.
Nws kuj raug pom zoo kom siv cov phau ntawv hluav taws xob nyob rau hauv qhov chaw ntawm cov ntawv sau phau ntawv ib txwm siv los khaws cov ncauj lus kom ntxaws ntxaws thaum sib koom ua ke hauv qhov system ib yam nkaus. Kev sib xyaw ua ke nrog cov phau ntawv siv hluav taws xob muab cov txheej txheem zoo sib xws ntau dua li phau ntawv sau classicallab.
3. Lab layout
Koj yuav tsum tsim koj qhov chaw kuaj mob kom ua kom zoo dua qub, thiab txhawb kev sib koom tes thiab yuav tsum ua kom qhov chaw muaj thiab teb rau koj cov neeg ua haujlwm xav tau.
Cais lub chaw kuaj sim rau hauv cov cheeb tsam sib txawv nrog ntau qib thiab hom kev phom sij, thiab npaj nyob ib puag ncig cov cheeb tsam ntawd. Cov cheeb tsam uas tau kwv yees tias yuav muaj "kev tsheb thauj neeg hnyav" yuav tsum tsis yog "qhov chaw muaj kev phom sij heev."
Tsim cov chaw sib txawv rau cov pej xeem thiab cov neeg ua haujlwm hauv chaw kuaj mob kom lawv tsis txhob sib tsoo. Muab cov cuab yeej siv loj nyob rau hauv qhov chaw xaiv tsa; lawv yuav tsum nyob deb ntawm qhov chaw tsheb loj tab sis nkag tau yooj yim.
4. Kev nyab xeeb
Thaum ntsuam xyuas qhov chaw kuaj mob peb yuav tsum txheeb xyuas qhov muaj feem cuam tshuam thiab teeb meem kev nyab xeeb tam sim ntawd. Xyuas kom txhua leej txhua tus raug teeb tsa rau kev cob qhia kev nyab xeeb yog qhov tsim nyog thiab aslo muaj ib lub rooj cob qhia rau koj cov neeg ua haujlwm hauv chaw kuaj mob uas qhia lawv txog kev phom sij ntawm lub chaw kuaj mob nrog rau cov txheej txheem thiab cov txheej txheem kev nyab xeeb kom raug.
Ua kom koj lub chaw kuaj mob muaj kev nyab xeeb nrog cov cuab yeej siv hauv paus, xws li lub tshuab tua hluav taws, daim pam hluav taws, cov khoom siv pab thawj zaug, da dej thaum muaj xwm ceev thiab hnab looj tes.
Xyuas kom meej tias kev nkag mus hauv chav kuaj yog txwv rau cov neeg ua haujlwm tsis tau tso cai los tiv thaiv ib qho xwm txheej thiab muaj ntau tshaj ib qho tawm ntawm lub chaw kuaj mob thaum muaj xwm txheej ceev.
5.Txhais khoom noj
Kev yuav khoom tseem ceeb yuav txawv ntawm chav kuaj mus rau chav kuaj, nyob ntawm qhov xav tau kev tshawb fawb. Txawm li cas los xij, nws tseem muaj txiaj ntsig zoo los koom tes nrog lwm tus thiab txheeb xyuas cov khoom xws li confocal microscopes thiab lwm yam khoom tshwj xeeb sib koom hauv cov chaw sim.
Thaum nws los txog rau qhov tseem ceeb, nws tsim nyog npog ntau lub hauv paus raws li koj tuaj yeem ua tau, txawm tias tsis muaj qhov xav tau tam sim ntawd. Ntawm no yog ib daim ntawv teev npe ntawm ntau lub hauv paus tag nrho cov chav kuaj yuav tsum yuav, segmented raws li qhov xav tau tshwj xeeb.
xws li:
Cell kab lis kev cai: Laminar flow hood, CO2 incubators, microscope nrog lub koob yees duab, tabletop txias centrifuge, mini centrifuge rau 1.5 mus rau 2 ml vials, dej da dej, nqus suction rau aspirate media, cell txee, kua nitrogen Dewars, cell freezing thawv, Petri tais diav thiab / lossis cov raj mis, thiab cov vials cryo nrog cov ntawv cim cryogenic.
Biochemistry thiab molecular biology: SDS-PAGE minipreps, western blot transfer apparatus, PCR thiab/lossis qPCR thermocyclers, sonicator, agarose gel apparatus, fais fab mov, DNA/RNA gel imager, Nanodrop lossis lwm txoj kev ntsuas DNA / RNA concentration, cov ntaub so ntswg homogenizer , phaj nyeem ntawv, pH meter, tshav kub thaiv, vortex, tshav kub thaiv, rotating shaker, centrifuges (rau 50 ml raj, microplates, 1.5-2 ml raj, thiab ib qho ultracentrifuge).
Cov khoom siv dav dav: Pipettes, cov lus qhia, tubing, racks, timers, txiab, iav, do tuav, thooj voos kheej kheej, Bunsen burner, fridges thiab freezers (4 ° C, -20 ° C, -80 ° C), sib npaug, hnab looj tes, carboys, tshuab xam zauv, autoclave bin, tapes, ntawv, tshuab luam ntawv, thiab cov cim.
Xa mus rau tsab xov xwm no: Ntau tshaj 20 lub chaw kuaj mob uas lawv siv
6. Cov ntaub ntawv.
Yuav tsum muaj ib lub roob ntawm cov ntaub ntawv, los ntawm kev ua HIRA (Hazard Identification and Risk Assessment) mus rau cov txheej txheem, thaum pib lub chaw kuaj mob tshiab. Nws yog qhov zoo dua los sab laj tus kws tshaj lij PI lossis tus kws cob qhia hauv koj daim teb uas tau dhau los ntawm kev teeb tsa lawv tus kheej lub chaw kuaj mob kom paub seb yuav ua li cas kom tau txais cov khoom txav hauv koj lub chaw kuaj mob. Tab sis yog tias koj tsis muaj cov ntaub ntawv tseem ceeb uas ntseeg tau, koj tuaj yeem ua koj tus kheej daim ntawv teev cov ntaub ntawv kom ua tiav los ntawm kev txheeb xyuas kom meej lub hom phiaj ntawm kev pib koj qhov chaw kuaj tshiab.
