Yuav ua li cas mus yuav cov khoom siv kuaj?

     Yuav ua li cas mus yuav cov khoom siv kuaj?

Lub hom phiaj ntawm kev yuav cov khoom siv hauv chav kuaj yog kom ntseeg tau tias cov khoom siv reagent siv hauv kev sim ruaj khov, txhim khu kev qha thiab pheej yig. Cov khoom siv feem ntau siv suav nrog: cov raj centrifuge, cov taub hau, cov raj kuaj, cov slides, pipettes thiab txhua yam reagents, yog li yuav tsum yuav cov khoom siv no li cas?

Ua ntej, ua tus tsim nyog kuaj cov khoom siv los ntawm lub luag haujlwm

1. Cov neeg ua hauj lwm lub luag hauj lwm rau consumables yuav tsum tam sim ntawd suav tag nrho consumables nyob rau hauv lub chaw soj nstuam, thov kev yuav khoom nyob rau hauv lub sij hawm, thiab nyob rau tib lub sij hawm coj kev txaus siab thaum tau txais consumables.
2. Cov kws tshuaj ntsuam xyuas yuav tsum ua raws li cov txheej txheem kev khiav hauj lwm nruj me ntsis, ua qhov kev ntsuam xyuas zoo ntawm cov khoom siv tau txais, thiab txiav txim siab qhov tshwj xeeb, rhiab heev, kev ua raws cai, thiab txo qis kev txwv ntawm cov tshuaj reagents rau kev tshawb nrhiav cov kab mob nucleic acids.

Dab tsi yog cov txheej txheem ua haujlwm rau kev yuav cov khoom siv hauv chav kuaj?

【Cov khoom siv】

1. Ua raws li cov kev cai tsim nyog, thiab cov khoom siv yuav tsum ua kom tiav nrog peb daim ntawv pov thawj (cov ntawv tso cai tsim khoom, daim ntawv pov thawj ntawm cov khoom lag luam, thiab daim ntawv tso cai ua lag luam).

Tus neeg saib xyuas lub chaw soj nstuam ua daim ntawv thov kev npaj rau kev yuav khoom siv raws li kev xav tau ntawm chav ua haujlwm thiab kev siv cov khoom siv tsis ntev los no, muab cov khoom siv thiab cov khoom muag ntawm lub hli dhau los, thiab ua tiav daim ntawv thov. kos npe. Kev muab tso ua ke thiab luam tawm yog uniformly yuav thiab tswj los ntawm lub koom haum.

2. Txais cov khoom siv

1) Kev tshuaj xyuas kev ntim khoom sab nraud:

Cov ntim khoom yuav tsum ua kom tiav, tsis muaj kev puas tsuaj, thiab tsis muaj kab mob, nrog rau daim ntawv qhia meej - lub chaw tsim khoom lub npe, cov khoom npe, tus naj npawb pom zoo, hnub tsim khoom, thiab hnub tas sij hawm.

2) Kev tshuaj xyuas sab hauv ntim:

Txawm hais tias lub ntim ntim sab hauv puas, lossis xau, seb cov ntsiab lus puas tiav, thiab seb puas muaj phau ntawv qhia sib raug.

3) Kev tshuaj xyuas saum toj no ua tiav thaum cov khoom tuaj txog, thiab cov ntaub ntawv tau ua tib lub sijhawm:

Khaws rau hauv qhov chaw cia khoom reagent.

3. Cov txheej txheem tshuaj xyuas zoo

1) Test centrifuge raj

Kev kuaj pom pom:

Xyuas seb lub centrifuge raj puas deformed, puas, thiab tsis tuaj yeem kaw.

Kev kuaj pom:

Cov raj centrifuge tuaj yeem dhau qhov kev lees paub tom qab kev kuaj pom.

Yog tias qhov kev tshuaj xyuas qhov muag tsis tsim nyog lossis tshwm sim, lossis txawm tias nquag tshwm sim tawg thiab tawg tom qab qhov kev tshuaj ntsuam pom dhau lawm, cov neeg ua haujlwm sim yuav tsum ua qhov kev sim tshuaj ntsuam xyuas.

1. Cov batch ntawm 30 random sampling centrifuge raj yog siv rau kev sim ntsuas.
2. Tom qab ntxiv 30 qhov pom kev centrifuge raj nrog ib nrab ntawm cov kua qaub ib txwm, centrifuge ntawm 10,000 rpm rau 20 feeb, yog tias nws pom tias lub hau ntawm lub raj tawg lossis xau, nws raug txiav txim siab tias batch ntawm centrifuge raj tsis ua raws li qhov no. yuav tsum tau kuaj kuaj yuav tsum tau rov qab ua tiav.
3. Tom qab qhov kev lees paub ua ntej, ua ntej cov batch ntawm centrifuge raj muab tso rau hauv kev ua haujlwm, coj ib qho, ntxiv 50 μL ntawm cov dej distilled, thiab ntsuas nrog cov qauv txhawm rau txheeb xyuas qhov paug ntawm cov batch ntawm centrifuge raj nrog cov khoom lag luam amplification.

Siv 10 lub raj centrifuge nyob rau hauv lub khob huv si nrog dej distilled, tsau rau 30 feeb, siv los kuaj cov batch ntawm centrifuge raj rau roj ester paug.

1. Tom qab qhov kev ntsuam xyuas saum toj no pom tsis muaj qhov tsis tsim nyog, batch ntawm centrifuge raj tuaj yeem qhib tau.

Nyob rau tib lub sijhawm, kom tsis txhob tawg ntawm lub centrifuge raj vim cua sov thaum lub sij hawm sim, ib phaj tau ntxiv rau cov cua sov module.

2) Tshawb nrhiav cov lus qhia

1. Xyuas seb lub hau yog deformed los yog puas.
2. Cov batch tau xaiv 30 lub tswv yim rau kev sim ntsuas.
3. Absorb incremental 0.1-0.2% glycerol ntxiv nrog tus cwj mem nrog tus qauv tsim los saib seb nws puas tau nqus mus rau hauv lub lim dej. Yog tias muaj, nws ua pov thawj tias cov khoom lim tau xau.
4. Aspirate tus nqi tsim nyog ntawm cov kua nrog ib tus qauv tsim nyog los saib seb puas muaj qhov txhaws qhov nqus lossis cov pa tawm. Tom qab tshem tawm cov khoom siv pipette, yog tias pom muaj qhov txawv txav, nws raug txiav txim siab tias batch ntawm cov lus qhia tsis tau raws li qhov kev sim ntsuas kev thov rov qab ua tiav.
5. Tom qab qhov kev ntsuam xyuas saum toj no tsis pom qhov xwm txheej tsis tsim nyog, koj tuaj yeem qhib lub batch ntawm consumables.
6. Txhua tus neeg ua haujlwm yuav tsum saib xyuas seb qhov zoo li cas yog raws li qhov yuav tsum tau ua thaum siv lossis faib cov khoom siv (piv txwv li, faib cov thawv ntim khoom).
7. Yog tias muaj teeb meem zoo nrog cov khoom siv, tam sim ntawd qhia rau tus neeg saib xyuas lub chaw soj nstuam hauv kev sau ntawv, thiab nrog kev pom zoo los ntawm tus thawj coj ntawm lub tuam tsev, ceeb toom rau lub tsev kho mob qhov chaw yuav khoom los tswj cov txheej txheem rov qab thiab pauv cov txheej txheem.
8. Kev ruaj khov ntawm cov khoom siv

6.1 Cov khoom siv ua ntej kev kho mob aseptic tau muab tso rau hauv thaj chaw reagent cia. Raws li kev ua haujlwm txhua hnub, tom qab kev kho kom muaj nuj nqis tsis tu ncua, lawv tau muab tso rau hauv thaj chaw npaj reagent, piv txwv qhov chaw npaj thiab nthuav dav thiab thaj chaw tshuaj xyuas khoom.

6.2 Cov khoom siv uas nquag siv tau ruaj khov thiab ntau muab tso rau hauv lub tub rau khoom ntawm lub rooj zaum hauv chav kuaj raws li kev ua haujlwm txhua hnub. Cov khoom siv tshwj xeeb tau muab tso rau hauv qhov chaw xaiv.

6.3 Cov koob tshuaj yuav tsum tau sau tseg thiab ntau npaum li cas siv yuav tsum tau ntxiv rau lub sijhawm.

6.4 Cov khoom siv iav yuav tsum muab tso rau hauv qhov chaw xaiv, thiab kev cia khoom yuav tsum tau ceev faj kom tsis txhob muaj kev puas tsuaj.

6.5 Ua ntej xaj khoom siv, yuav tsum tau kuaj xyuas kom raug.

【Reagents】

1. Txhua lub chaw ua ntawv thov rau kev yuav cov tshuaj reagents yuav tsum xa ib daim ntawv thov. Tom qab lub tuam tsev pom zoo, xa mus rau lub tuam tsev kho mob thiab cov thawj coj hauv chav ua haujlwm kom pom zoo thiab yuav khoom.
2. Yuav kom yuav reagents, lub npe reagent, chaw tsim tshuaj paus, hnub tim ntawm kev yuav khoom, batch naj npawb, hnub tas sij hawm, kom muaj nuj nqis, thiab lwm yam. Tus neeg tshwj xeeb khaws daim ntawv sau npe.
3. Kev khaws cia ntawm cov reagents yuav tsum tau muab cais raws li qhov sib txawv ntawm cov kev xav tau ntawm ntau yam reagents, thiab kev sau npe yuav tsum tau ua.
4. Kev tswj xyuas zoo ntawm txhua pawg ntawm reagents, suav nrog reagent yin-yang tswj thiab paub cov qauv yin-yang tswj thiab cov ntaub ntawv.
5. Cov reagents uas ua rau nws tus kheej yuav tsum sau npe, qauv, ntau, hnub tim, thiab lwm yam, thiab txhua pawg ntawm reagents yuav tsum tau tswj kom zoo.
6. Thaum siv cov tshuaj reagents, kev tswj kom zoo yuav tsum tau ua tsis tu ncua lossis tsis xwm yeem. Yog tias cov reagents pom tias muaj qhov tsis txaus ntseeg, lawv yuav tsum raug tso tseg tam sim ntawd txhawm rau nrhiav qhov laj thawj.
7. Performance check ntawm cov khoom siv:

Ua qhov kev soj ntsuam zoo ntawm kev ua haujlwm ntawm cov khoom siv thiab siv lub phaj dej rau kev sim. Siv cov khoom siv los kuaj xyuas los txiav txim siab ntau yam qauv hauv cov khoom siv tshuaj ntsuam xyuas, nqa cov tais ntim ntshav raws li tus qauv, thiab suav cov rhiab heev, qhov tshwj xeeb thiab qhov sib txawv ntawm cov khoom siv kuaj raws li cov lus hauv qab no thiab cov qauv txuas nrog.

1) Ib tus lej ntawm cov qauv tsis zoo thiab 3 txog 5 qhov kev kuaj pom zoo, tag nrho cov qauv yog 20.

2) Ua qhov kev sim ua raws li cov lus qhia los ntawm cov khoom siv. Kuaj qhov tshwj xeeb, rhiab heev thiab ua raws li cov khoom siv; serial dilution specimens tuaj yeem txiav txim siab qis qis ntawm cov reagent.

Qhov tshwj xeeb (%) = D / (B + D) * 100%

Sensitivity (%) = A / (A + C) * 100%

Tus nqi sib tw (%) = A + D / (A + B + C + D) * 100%

Nyob rau hauv cov xwm txheej zoo tagnrho, qhov tshwj xeeb, rhiab heev thiab qhov sib txawv ntawm cov khoom siv yog 100%.

3) Yog tias muaj teeb meem zoo nrog cov reagents thiab consumables, tam sim ntawd qhia rau tus neeg saib xyuas nyob rau hauv kev sau ntawv thiab ceeb toom rau lub tsev kho mob chaw muab kev pab nyob rau hauv kev sau ntawv los lis kev xa rov qab thiab hloov cov txheej txheem.

8. Test reagents yog khaws cia raws li kev cia khoom