How to buy laboratory consumables?

Laboratory glassware type and classification management

     How to buy laboratory consumables?

The purpose of purchasing laboratory consumables is to ensure that the reagent consumables used in the experiment are stable, reliable and economical. Commonly used consumables include: centrifuge tubes, tips, test tubes, slides, pipettes and all reagents, so how should these consumables be purchased?

First, be a qualified laboratory consumable buyer from the duty

  1. The staff responsible for consumables should promptly count all consumables in the laboratory, make purchase requests at regular intervals, and at the same time conduct acceptance when receiving consumables.
  2. Laboratory inspectors must strictly abide by the operating procedures, perform quality tests on the received kits, and determine the specificity, sensitivity, compliance rate, and lower detection limits of the reagents for the detection of pathogen nucleic acids.

What are the working procedures for purchasing laboratory consumables?


  1. Strictly follow the relevant regulations, and the consumables should be complete with three certificates (production license, product registration certificate, business license).

The person in charge of the laboratory makes an application plan for purchasing consumables based on the work needs of the room and the recent consumption of consumables, and provides the consumption and inventory of the previous month, and fills out the requisition form. signature. The compilation and printing are uniformly purchased and managed by the organization.

  1. Acceptance of consumables

1) Outer packaging inspection:

The packaging should be complete, non-destructive, and non-contaminating, with a clear label—the manufacturer’s name, product name, approval number, production date, and expiration date.

2) Inner packaging inspection:

Whether the inner packaging is damaged, leaked, whether the content is complete, and whether there is a corresponding instruction manual.

3) The above inspection is completed when the goods arrive, and records are made at the same time:

Stored in the reagent storage area.

  1. Quality inspection procedures

1) Test centrifuge tube

Visual inspection:

Check whether the centrifuge tube is deformed, damaged, and cannot be closed.

Experimental detection:

The centrifuge tubes can basically pass the acceptance after the visual inspection.

If the visual inspection is unqualified or occurs, or even frequently occurs bursts and leaks after the visual inspection is passed, the experimental personnel shall conduct the experimental inspection.

  1. The batch of 30 random sampling centrifuge tubes is used for experimental testing.
  2. After adding 30 visually qualified centrifuge tubes with half the amount of normal saline, centrifuge at 10,000 rpm for 20 minutes, if it is found that the cap of the tube bursts or leaks, it is considered that the batch of centrifuge tubes does not meet this requirement Laboratory experiments require that returns be processed.
  3. After preliminary acceptance, before the batch of centrifuge tubes is put into operation, take one, add 50 μL of distilled water, and measure with the specimen to detect the contamination of the batch of centrifuge tubes with amplification products.

Take 10 centrifuge tubes in a clean beaker with distilled water, soak for 30 minutes, used to test the batch of centrifuge tubes for oil ester contamination.

  1. After the above test finds no unqualified conditions, the batch of centrifuge tubes can be activated.

At the same time, in order to avoid the explosion of the centrifuge tube due to heating during the experiment, a plate was added to the heating module.

2) Detection tip

  1. Check if the tip is deformed or damaged.
  2. The batch randomly selected 30 tips for experimental testing.
  3. Absorb incremental 0.1-0.2% glycerol added with ink with a suitable sampler to see if it is sucked into the filter element. If there is, it proves that the filter element is leaking.
  4. Aspirate an appropriate amount of liquid with a suitable sampler to see if there are clogged suction holes or air leaks. After removing the pipette factor, if any abnormality is found, it is considered that the batch of tips does not meet the laboratory experiments Request for return processing.
  5. After the above test does not find the unqualified situation, you can activate the batch of consumables.
  6. Every staff member must observe whether the appearance is in accordance with the requirements when using or distributing consumables (for example, distributing collection containers).
  7. If there are quality problems with consumables, immediately report to the person in charge of the laboratory in writing, and with the informed consent of the director of the department, notify the hospital procurement center to handle the return and exchange procedures.
  8. Storage of consumables

6.1 The consumables before aseptic treatment are placed in the reagent storage area. According to the daily workload, after regular quantitative treatment, they are placed in the reagent preparation area, sample preparation area and amplification and product analysis area.

6.2 Commonly used consumables are fixedly and quantitatively placed in the drawer of the laboratory bench according to the daily workload. Special consumables are placed in designated places.

6.3 The dosage shall be recorded and the quantity used shall be supplemented in time.

6.4 Glass supplies should be placed in designated locations, and storage should be careful to avoid damage.

6.5 Before ordering consumables, the quantity should be checked accurately.



  1. Each department applying for the purchase of reagents should submit a written application. After the department agrees, submit it to the medical department and unit leaders for approval and purchase.
  2. To purchase reagents, the reagent name, manufacturer, date of purchase, batch number, expiration date, quantity, etc. should be registered. Special person keeps the registration.
  3. The storage of reagents should be stored separately according to the different requirements of various reagents, and registration should be done.
  4. Quality control of each batch of reagents, including reagent yin-yang control and known specimen yin-yang control and record.
  5. Self-produced reagents should register the name, formula, quantity, date, etc., and each batch of reagents should be quality controlled.
  6. During the use of reagents, quality control should be carried out regularly or irregularly. If the reagents are found to be suspicious, they should be stopped immediately to find the reason.
  7. Performance check of the kit:

Perform quality inspection on the performance of the kit and use a serum plate for testing. Use the kit to be tested to determine the various specimens in the serum tray, take the serum tray as the standard, and calculate the sensitivity, specificity and coincidence rate of the tested kit according to the following table and the attached formula.

1) A certain number of negative samples and 3 to 5 serially diluted positive samples, the total number of samples is 20.

2) Perform the experiment according to the instructions provided by the kit. Test the specificity, sensitivity and conformity of the kit; serial dilution specimens can determine the lower limit of the reagent.

Specificity (%)=D/(B+D)*100%

Sensitivity (%)=A/(A+C)*100%

Accordance rate (%)=A+D/(A+B+C+D)*100%

Under ideal conditions, the specificity, sensitivity and coincidence rate of the kit are 100%.

3) If there are quality problems with reagents and consumables, immediately notify the person in charge in writing and inform the hospital procurement center in writing to handle the return and replacement procedures.

  1. Test reagents are stored according to storage conditions


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